TABLE 4.
Variable nucleotides in the β-tubulin gene fragment amplified from seven isolates of T. weissflogii
| Isolatea | Nucleotides at the following positionsb:
|
|||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intron
|
Coding
|
|||||||||||||||||||||||||||||||
| 42 | 46 | 55 | 56 | 57 | 68 | 76 | 77 | 79 | 81 | 85 | 86 | 87 | 90 | 91 | 109 | 127 | 174 | 180 | 204 | 231 | 333 | 408 | 411 | 417 | 444 | 483 | 510 | 579 | 585 | 621 | 627 | |
| Long Island Sound clone Actin | A | C | — | — | G | G | C | C | G | A | T | C | G | A | T | C | A | C | A | C | T | A | T | C | G | T | T | C | G | C | A | G |
| Long Island Sound clone 4C | . | . | — | — | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| Norway | . | . | — | — | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| Portugal | . | . | — | — | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
| South Pacific | C | — | T | A | A | C | G | G | T | T | . | G | A | G | . | T | G | A | T | T | C | T | C | T | A | C | C | T | A | T | T | C |
| California | C | — | T | A | A | C | G | G | T | T | . | G | A | G | . | T | G | A | T | T | C | T | C | T | A | C | C | T | A | T | T | C |
| Hawaii | C | — | T | A | . | C | G | G | T | T | C | G | A | G | . | T | G | . | T | T | C | T | C | T | A | C | C | T | A | T | T | C |
Long Island Sound clones Actin and 4C and the Norway and Portugal isolates were obtained from the Atlantic Ocean, and the South Pacific, California, and Hawaii isolates were obtained from the Pacific Ocean basin.
The positions are numbered relative to the first nucleotide immediately following the forward primer. A dot indicates that the nucleotide is identical to the nucleotide in the Long Island Sound clone Actin reference clone; dashes indicate gaps.