TABLE 5.
Variable nucleotides present in two or more copies of the Sig1 gene fragment amplified from seven isolates of T. weissflogii
Isolatea | Clone | Nucleotides present in two or more copies at the following positionsb:
|
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---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Coding
|
Intron
|
Coding Coding
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
1 | 19 | 25 | 28 | 31 | 53 | 64 | 65 | 66 | 85 | 86 | 91 | 98 | 102 | 114 | 119 | 121 | 132 | 140 | 161 | 189 | 196 | 198 | 211 | 212 | 228 | 229 | 234 | 237 | 241 | 267 | 274 | 297 | 315 | 318 | 335 | 345 | 387 | 405 | 417 | 435 | 443 | 483 | 531 | 534 | 558 | 579 | 596 | 605 | 618 | 630 | 632 | 638 | 639 | ||
Long Island Sound clone Actin | Reference | A | C | T | G | C | T | T | — | — | A | T | A | G | T | A | C | C | T | C | A | T | G | G | G | G | A | A | A | T | T | C | T | T | C | G | A | T | T | A | T | A | C | G | C | A | A | C | T | A | C | T | A | T | C |
1 | C | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | A | . | . | C | . | . | . | . | . | . | . | . | . | |
2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | ||
3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | C | . | . | . | . | . | . | . | . | . | A | . | . | C | . | . | . | . | . | . | . | . | ||
4 | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |||
5 | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | |
6 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
7 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
8 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
9 | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | A | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | |
10 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
Long Island Sound clone 4C | 1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . |
2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | |
3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | |
5 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | |
Norway | 1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | C | . | . | T | A | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | T | A | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
5 | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | A | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
Portugal | 1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |||
5 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
California | 1 | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |
2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
5 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
South Pacific | 1 | . | T | . | A | T | C | C | G | C | T | . | T | A | G | . | T | . | C | T | . | . | . | A | . | . | T | G | G | C | . | G | . | . | T | A | . | C | G | C | C | G | C | A | T | . | . | A | A | G | T | C | . | C | A |
2 | . | T | . | A | T | C | C | G | C | C | . | T | A | G | . | T | . | C | T | . | . | . | A | . | . | T | G | G | C | . | G | . | . | T | . | . | C | G | C | C | G | . | A | T | . | . | A | A | G | T | C | . | C | A | |
3 | . | T | . | A | T | C | C | G | C | T | . | T | A | G | . | T | . | C | T | . | . | . | A | . | . | T | G | G | C | . | G | . | . | T | A | . | C | G | C | C | G | . | A | T | . | . | A | A | G | T | C | . | C | A | |
4 | . | T | . | A | T | C | C | G | C | T | . | T | A | G | . | T | G | C | T | G | . | . | A | . | . | T | G | G | C | . | G | . | . | T | A | . | C | G | C | C | G | . | A | T | . | . | A | A | G | T | C | . | C | A | |
5 | . | T | . | A | T | C | C | G | C | T | . | T | A | G | . | T | G | C | T | G | . | . | A | . | . | T | G | G | C | . | G | . | . | T | A | . | C | G | C | C | G | . | A | T | . | . | A | A | G | T | C | . | C | A | |
Hawaii | 1 | . | T | . | A | T | C | C | . | . | T | . | T | A | G | T | T | . | C | . | . | . | . | . | . | . | T | G | G | C | . | G | . | C | T | A | . | C | G | C | C | G | . | A | T | . | G | A | A | . | T | C | . | C | A |
2 | . | T | . | A | T | C | C | . | . | T | . | T | A | G | T | T | . | C | . | . | . | . | . | . | . | T | G | G | C | . | G | . | C | T | A | . | C | G | C | C | G | . | A | T | . | G | A | A | . | T | C | . | C | A | |
3 | . | T | . | A | T | C | C | . | . | T | . | T | A | G | T | T | . | C | . | . | . | . | . | . | . | T | G | G | C | . | G | . | C | T | A | . | C | G | C | C | G | . | A | T | . | G | A | A | . | T | C | . | C | A | |
4 | . | T | . | A | T | C | C | . | . | T | . | T | A | G | T | T | . | C | . | . | . | . | . | . | . | T | G | G | C | . | G | . | C | T | A | . | C | G | C | C | G | . | A | T | . | G | A | A | . | T | C | . | C | A | |
5 | . | T | . | A | T | C | C | . | . | T | . | T | A | G | T | T | . | C | . | . | . | . | . | . | . | T | G | G | C | . | G | . | C | T | A | . | C | G | C | C | G | . | A | T | . | G | A | A | . | T | C | . | C | A |
Long Island Sound Clones Actin and 4C and the Norway and Portugal isolates were obtained from the Atlantic Ocean, and the California, South Pacific, and Hawaii isolates were obtained from the Pacific Ocean basin.
The positions are numbered relative to the first nucleotide immediately following the forward primer. A dot indicates that the nucleotide is identical to the nucleotide in the Long Island Sound Clone Actin reference clone; dashes indicate gaps.