Table 3.
Reagents Used
| Reagents | Company | Catalog number |
|---|---|---|
| L-WRN cells | ATCC (Manassas, VA) | CRL-3276 |
| Advanced DMEM/F12 | Thermo Fisher (Waltham, MA) | 12634010 |
| Glutamax, 100× | Thermo Fisher (Waltham, MA) | 35050061 |
| 1 mol/L HEPES buffer | Corning (Corning, NY) | 25-060-CI |
| Primocin | VWR (Radnor, PA) | MSPP-ANTPM2 |
| N-acetyl-cysteine | MilliporeSigma (Burlington, MA) | A9165 |
| EGF, murine | Peprotech (Rocky Hill, NJ) | 315-09 |
| Nicotinamide | MilliporeSigma (Burlington, MA) | N0636-100G |
| B27 | Thermo Fisher (Waltham, MA) | 12587-010 |
| Gastrin | MilliporeSigma (Burlington, MA) | G9145-1MG |
| PGE2 | Peprotech (Rocky Hill, NJ) | 3632464 |
| A 83-01 | MilliporeSigma (Burlington, MA) | SML0788-5MG |
| SB202190 | Peprotech (Rocky Hill, NJ) | 1523072 |
| DPBS | Thermo Fisher (Waltham, MA) | 14190-144 |
| Matrigel | Corning (Corning, NY) | 354230 |
| Y27632 | Selleckchem (Houston, TX) | S6390 |
| Etomoxir | MilliporeSigma (Burlington, MA) | E1905-5MG |
| C75 | Cayman Chemical (Ann Arbor, MI) | 10005270 |
| Metformin | Cayman Chemical (Ann Arbor, MI) | 13118 |
| Dimethyl sulfoxide | MilliporeSigma (Burlington, MA) | D2650-5X5ML |
| Glyoxal solution 40 wt % in H2O | MilliporeSigma (Burlington, MA) | 128465 |
| TrypLE Express | Thermo Fisher (Waltham, MA) | 12605010 |
| Dextran 647 | Thermo Fisher (Waltham, MA) | D22914 |
| a-BODIPY 530/550-C12-HPC | Thermo Fisher (Waltham, MA) | D3792 |
| BODIPY FL C16 | Thermo Fisher (Waltham, MA) | D-3821 |
| BODIPY FL C12 | Thermo Fisher (Waltham, MA) | D-3822 |
| BODIPY-FL C5 | Thermo Fisher (Waltham, MA) | D-3834 |
| BODIPY 493/503 | Thermo Fisher (Waltham, MA) | D-3922 |
| 18:1-18:1-C11 TopFluor TG | Avanti Polar Lipids (Birmingham, AL) | 810298C-1mg |
| Na2HPO4 | MilliporeSigma (Burlington, MA) | S7907 |
| KH2PO4 | MilliporeSigma (Burlington, MA) | P5655 |
| NaCl | MilliporeSigma (Burlington, MA) | S5886 |
| KCl | MilliporeSigma (Burlington, MA) | P5405 |
| Sucrose | Thermo Fisher (Waltham, MA) | BP 220-1 |
| D-sorbitol | Thermo Fisher (Waltham, MA) | BP439-500 |
| EDTA | Corning (Corning, NY) | 46-034-Cl |
| DTT | Thermo Fisher (Waltham, MA) | BP172-5 |
| Protease VIII | MilliporeSigma (Burlington, MA) | P5380 |
| Bovine serum albumin | Thermo Fisher (Waltham, MA) | BP1600-1 |
| TotalSeq-B0251 anti-human hashtag 1 antibody | BioLegend (San Diego, CA) | 394631 |
| TotalSeq-B0251 anti-human hashtag 2 antibody | BioLegend (San Diego, CA) | 394633 |
| TotalSeq-B0251 anti-human hashtag 3 sntibody | BioLegend (San Diego, CA) | 394635 |
| TotalSeq-B0251 anti-human hashtag 4 antibody | BioLegend (San Diego, CA) | 394637 |
| TotalSeq-B0251 anti-human hashtag 5 antibody | BioLegend (San Diego, CA) | 394639 |
| TotalSeq-B0251 anti-human hashtag 6 antibody | BioLegend (San Diego, CA) | 394641 |
| Annexin V-APC | BioLegend (San Diego, CA) | 640920 |
| RNAqueous-Micro Total RNA Isolation Kit | Thermo Fisher (Waltham, MA) | AM1931 |
| Chromium Next GE M Single-Cell 3’ GEM, Library and Gel Bead Kit v3.1 | 10x Genomics (Pleasanton, CA) | PN-100012 |
| RNAscope Probe - Hs-FABP1-C3 | Advanced Cell Diagnostics (Newark, CA) | 534801-C3 |
| RNAscope Probe - Hs-APOA4 | Advanced Cell Diagnostics (Newark, CA) | 857841 |
| RNAscope Probe - Hs-OLFM4-C2 | Advanced Cell Diagnostics (Newark, CA) | 311041-C2 |
| RNAscope Probe - Hs-DMBT1 | Advanced Cell Diagnostics (Newark, CA) | 478711 |
DTT, dithiothreitol; EGF, epidermal growth factor; HPC, 1-Hexadecanoyl-sn-Glycero-3-Phosphocholine L-WRN, L-cell derived Wnt3a, R spondin 3, and Noggin; PGE2, prostaaglandin E2.