Figure 5.

Characterizing putative interaction regulatory regions. (A) Volcano plot showing interaction log₂ fold change versus –log₁₀P value for lenient (P value <.01, >15 base mean; red) cutoffs identifies H3K27ac regulatory windows with greatest potential for interaction. Twenty thousand out of 547,000+ enriched H3K27ac windows that did not pass the lenient interaction cutoff were chosen at random to represent noninteracting sites. (B) Scatterplot comparing H3K27ac log₂ interaction windows fold change linked to the RNA log₂ interaction fold change for genes that also show interaction reveals a positive correlation suggesting many of these regions are causal in contributing to the transcription patterns of these genes across +CV and +HFM conditions. (C) Selected putative H3K27ac interaction windows from each cluster showing consistent patterns of H3K27ac enrichment and relative RNA levels across the 4 conditions.