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. 2022 May 13;14(2):494–511. doi: 10.1016/j.jcmgh.2022.05.002

Figure 2.

Figure 2

Effects of IE2 expression on hepatoblasts and hepatocytes during liver development. (A) Immunofluorescence at stages E15.5, E17.5, and D1 livers, with primary antibodies against IE2 (red), DLK1 (red), AFP (green), ALB (green), and Alexa Fluor 488– and 555–conjugated secondary antibodies. Scale bars: 100 μm. (B) Quantification of IE2-, DLK1-, AFP-, and ALB-positive cells from panel A. n = 3–5 mice per group. Error bars represent SEM. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001. n = 3–5 mice per group. NS, no statistical difference.