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. 2022 May 13;14(2):494–511. doi: 10.1016/j.jcmgh.2022.05.002

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

IE2-specific expression promotes cell apoptosis. (A) The cell apoptosis was detected by flow cytometry between D1 Rosa26-LSL-IE2^+/- and Rosa26-LSL-IE2^+/-, cre mice. (B) Proportional distribution of fluorescein isothiocyanate (FITC)+ PI+ cells. (C) mRNA expression levels of the apoptosis-related genes were measured by quantitative real-time PCR. (D) Immunofluorescence for E15.5, E17.5, and D1 livers, with primary antibodies against IE2 (red), caspase 3 (green), and Alexa Fluor 488– and 555–conjugated secondary antibodies. Error bars represent SEM. The Student t test was used to analyze statistical differences. ∗∗P < .01, ∗∗∗P < .001. n = 3–5 mice per group. DAPI, 4′, 6-diamidino-2-phenylindole.