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. 2022 May 13;14(2):494–511. doi: 10.1016/j.jcmgh.2022.05.002

Figure 7.

Figure 7

The inflammatory milieu in Rosa26-LSL-IE2+/-, cre livers. (A) Heatmap representing expression of genes related to an inflammatory response. (B) mRNA expression levels of IL1β, IL6, and TNFα were measured by quantitative real-time PCR. (C) H&E showed scattered inflammatory cell infiltration (red arrows) in Rosa26-LSL-IE2+/-, cre livers. Scale bars: 20 μm. (D) The percentage of CD4+ and CD8+ T cells in hepatic CD3+ T cells was detected by flow cytometry. The cells were analyzed by a fluorescence-activated cell sorter. (E) Summarized data of panel D. (F) Immunofluorescence for D1 livers, with primary antibodies against CD3 (green), CD4 (red), CD8 (pink), and Alexa Fluor 488– and 555–conjugated secondary antibodies. Scale bars: 20 μm. Error bars represent SEM. The Student t test was used to analyze statistical differences. ∗P < .05, ∗∗P < .01. n = 3–5 mice per group. SSC, side scatter.