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. 2022 May 13;14(2):494–511. doi: 10.1016/j.jcmgh.2022.05.002

Figure 8.

Figure 8

Inadequate maturation of remnant hepatocytes in Rosa26-LSL-IE2+/-, cre livers. (A) Hepatocellular cytoplasmic glycogen stores stained with periodic acid–Schiff. Scale bars: 20 μm. (B) Immunofluorescence for zonula occludens-1 (ZO-1) showed tight junctions in the D1 livers. Scale bars: 20 μm. (C) GSEA showing target metabolism pathways. Heatmap representing alteration of gene expression enriched in hepatocyte differentiation and maturation. Green represents a down-regulation in expression and red represents up-regulation when compared with the Rosa26-LSL-IE2+/- group. (D) mRNA expression levels of the major cell markers and important transcription factors were measured by quantitative real-time PCR. (E) The protein expression levels of CEBPα and HNF4α were measured by Western blot (left). Quantification of CEBPα and HNF4α expression (right). Ctrl represents Rosa26-LSL-IE2+/-, IE2 represents Rosa26-LSL-IE2+/-, cre. Error bars represent SEM. The Student t test was used to analyze statistical differences. ∗∗P < .01, ∗∗∗P < .001. n = 3–5 mice per group. Ctrl, control; DAPI, 4′, 6-diamidino-2-phenylindole.