Skip to main content
. 2022 May 13;14(2):435–464. doi: 10.1016/j.jcmgh.2022.05.003

Figure 1.

Figure 1

Wnt mimetics alone repaired the damaged colon epithelium in an acute DSS colitis model. (A) A diagram illustrating the acute DSS model used. DSS was included in drinking water at the indicated percentage. Wnt mimetics and RSPO were dosed twice on day 4 and day 7 (yellow arrows) or daily starting on day 4. Animals were terminated on day 10. (Animals were switched to plain drinking water on the last day for compliance with Institutional Animal Care and Use Committee protocol.) (B–D) H&E stain of transverse colon sections of normal colon and the damaged colon on day 4 and day 7. (F–Q) RNAscope in situ showing expression of Axin2, Lgr5, Rnf43, Wnt2b, Wnt5a, and Rspo3 in normal and DSS colon tissues. (R–Y) Representative H&E stain of transverse colon of the indicated treatment groups: (R) no DSS, (S) DSS with PBS, (T) with 2 treatments of 3 mg/kg anti-GFP, (U) with 2 treatments of 10 mg/kg R2M3-26, (V, W) with 2 or daily treatments of 3 mg/kg RSPO2, and (X, Y) with 2 or daily treatments of 1 mg/kg RSPO2 + 0.3 mg/kg R2M3-26 combo. (Z) Colon histology score of acute DSS conditions after the indicated treatments. Statistical analysis was performed as described in the Methods with all comparisons made to the anti-GFP group. Scale bars = 200 μm.