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. 2022 May 13;14(2):435–464. doi: 10.1016/j.jcmgh.2022.05.003

Figure 11.

Figure 11

DSS injury induces specific inflammatory cell types/states in the epithelium. (A) Row-scaled Z scores for each gene across the aggregate of each cell type in the epithelium. The color bar corresponds to the cell type. (B) Selected top gene sets or pathways enriched in the epithelium at day 5 upon DSS damage determined by GSEA. (C) Selected top gene sets enriched in the AltEnteros injuryiinduced cell type at day 5 (24 hours) relative to the other cell types in the epithelium. (D) The distribution of gene expression for the 2 indicated gene sets in the AltEnteros. (E) (top left) The log2-normalized expression of Krt20 and Ly6a in the epithelial cells showing coexpression of high levels of Ly6a with Krt20 in the DSS injury conditions. (Top right) t-SNE plot colored by Ly6a expression in the epithelial cells. (Bottom left) The log2-normalized values showing coexpression of Krt20 and Ly6c1 in the epithelial cells. (Bottom right) t-SNE plot of the epithelial cells colored by the expression of Ly6c1. (F) RNA in situ of the enterocyte marker, Krt20, and Ly6a. Scale bar = 100 mm. (G) FACS plots showing the degree of enrichment of EPCAM-positive, LY6A-positive cells (upper right quadrant of each plot) in the uninjured condition (left) or upon 4 days of DSS damage (right).