Skip to main content
. 2022 May 13;14(2):435–464. doi: 10.1016/j.jcmgh.2022.05.003

Figure 4.

Figure 4

In vitro activity of Fzd5,8 subfamily–specific Wnt mimetic SZN-1326-p. (A) The binding affinity of the Fzd5,8 binder IgG of SZN-1326-p to its target Fzd5 CRD measured on Octet. equilibrium dissociation constant (KD) indicated on the graph. (B) The binding affinity of the Fzd5,8 binder IgG of SZN-1326-p to its target Fzd8 CRD measured on Octet. KD indicated on the graph. (C) The binding specificity of the Fzd5,8 binder IgG of SZN-1326-p to each of the 10 Fzd CRDs examined on Octet. (D) The dose-dependent STF activities of SZN-1326-p, of the Fzd1,2,7-specific mimetic 1RC07-26 and of the Fzd1,2,5,7,8 pan specific mimetic R2M3-26 in the presence of 20 nM RSPO2 measured in Huh-7 cells. RLU, relative luminescence units.