Table 1.
Reagents and Resources
| Reagent or Resource | Source | Identifier |
|---|---|---|
| ACD probes | ||
| RNAscope Probe—Mm-Axin2 | ACD #400331 | |
| RNAscope Probe—Mm-Lgr5 | ACD #312171 | |
| RNAscope Probe—Mm-Rnf43 | ACD #400371 | |
| RNAscope Probe—Mm-Wnt2b | ACD #405031 | |
| RNAscope Probe—Mm-Wnt5a | ACD #316791 | |
| RNAscope Probe—Mm-RSPO3 | ACD #402011 | |
| RNAscope Probe—Mm-Fzd1 | ACD #404871 | |
| RNAscope Probe—Mm-Fzd2 | ACD #404881 | |
| RNAscope Probe—Mm-Fzd3 | ACD #404891 | |
| RNAscope Probe—Mm-Fzd4 | ACD #404901 | |
| RNAscope Probe—Mm-Fzd5 | ACD #404911 | |
| RNAscope Probe—Mm-Fzd6 | ACD #404921 | |
| RNAscope Probe—Mm-Fzd7 | ACD #404931 | |
| RNAscope Probe—Mm-Fzd8 | ACD #404941 | |
| RNAscope Probe—Mm-Fzd9 | ACD #404951 | |
| RNAscope Probe—Mm-Fzd10 | ACD #315781 | |
| RNAscope Probe—Mm-Krt20 | ACD #402301 | |
| RNAscope Probe—Mm-Ly6a | ACD #427571 | |
| RNAscope Probe—Mm-Pdgfra | ACD #480661 | |
| RNAscope Probe—Mm-Ccl8 | ACD #546211 | |
| RNAscope Probe—Mm-Cdkn3 | ACD #401701 | |
| Antibodies, enzymatic kits | ||
| Rabbit anti-Villin (SP145) | Abcam | ab130751 |
| Rabbit anti-DCLK/DCAMKL1 (D2U3L) | Cell Signaling Technology | CST 62257 |
| Rabbit anti-chromogranin A | Abcam | ab15160 |
| Rabbit anti-ZO-1 (clone 1A12) | Thermo Fisher Scientific | 33-9100 |
| Rabbit anti-Ki67 | Abcam | 15580 |
| Rat anti-KI67 (clone SolA15) | Thermo Fisher Scientific | 14-5698-82 |
| Rat anti-EPCAM-Alexa-488 (clone G8.8) | BioLegend | 118210 |
| Rat anti-LY6A-Alexa-647 (clone E13-161.7) | BioLegend | 122518 |
| Rat IgG2 Isotype control-Alexa-488 | BioLegend | 400525 |
| FcR blocking Reagent | Miltenyi Biotec | 130-092-575 |
| Donkey anti-rat IgG (H&L), highly cross-adsorbed secondary antibody, Alexa Fluor 488 | Thermo Fisher Scientific | A-21208 |
| Anti-Green Fluorescence Protein (Anti-GFP) human IgG | Surrozen | |
| hFc-RSPO2 | Surrozen | |
| R2M3-26, bi-specific appended human IgG effector-less format | Surrozen | |
| SZN-1326-p, bi-specific appended human IgG effector-less format (parental molecule of a clinical candidate SZN-1326) | Surrozen | |
| 1RC07-26, bi-specific appended human IgG effector-less format | Surrozen | |
| RNAscope 2.5 HD Assay-Red | ACD Bio | |
| RNAscope Mulitplex Fluorescent Reagent Kit, v2 Assay | ACD Bio | |
| Zymo Direct-zol RNA Microprep | Zymo | R2062 |
| MagMAX mirVana Total RNA Isolation Kit | Thermo Fisher Scientific | A27828 |
| Applied Biosystems High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | 4368814 |
| Applied Biosystems TaqMan Fast Advanced Master Mix | Thermo Fisher Scientific | 4444557 |
| Chemicals, peptides, proteins | ||
| DMEM/F12 | Thermo Fisher Scientific | 12634-010 |
| DAPI | Thermo Fisher Scientific | D1306 |
| Fetal bovine serum | Thermo Fisher Scientific | 10438-026 |
| Liberase | Sigma | 05401127001 |
| DNAse1 | Sigma | 04716728001 |
| EDTA | Sigma | 03609 |
| Phosphate-buffered saline | Thermo Fisher Scientific | 10010-023 |
| HEPES | Thermo Fisher Scientific | J16924-AE |
| Sodium pyruvate | Thermo Fisher Scientific | 11360-070 |
| Pen-Strep | Thermo Fisher Scientific | 15140-122 |
| Antibiotic/antimycotic 100× | Thermo Fisher Scientific | 15240-062 |
| Hank’s Balanced Salt Solution | Thermo Fisher Scientific | 14175-079 |
| TrypLE | Thermo Fisher Scientific | 12604-013 |
| Triton X-100 | ACROS Organics | 21568-2500 |
| TSA Plus Cyanine 3 System | Akoya Bioscience | NEL744001KT |
| TSA Plus cyanine 5 System | Akoya Bioscience | NEL745001KT |
| Vectashield Vibrance antifade mounting medium with DAPI | Vector Laboratories | H-1800 |