Fig. 8. In vivo testing of a trivalent NA-MPP vaccine.
Female, 6–8 week old BALB/c mice (n = 5 per group) were vaccinated in a prime/boost regimen with the respective antigens. Blood was obtained 42 days after the prime and used for serological analysis. A Weight loss and B survival curve after challenge with 25xmLD50 of A/Singapore/GP1908/15 H1N1 (IVR-180). Differences in survival were analyzed using a Mantel–Cox log-rank test. N1-MPP vs Neg. Ctrl. p = 0.0143; all other vaccinated groups vs Neg. Ctrl. p = 0.0027; other differences were not statistically significant (p > 0.05). C Weight loss and D survival curve after challenge with 5xmLD50 of A/Vietnam/1203/04 H5N1 (6:2 A/Puerto Rico/8/34 reassortant, polybasic cleavage site removed). Differences in survival were analyzed using a Mantel–Cox log-rank test. N1 + N2 + B-NA-MPP + ODN1018 vs. Neg. Ctrl. (p = 0.0116) was statistically significant, the differences between remaining curves were not statistically significant (p > 0.05). D, E ELISA against rec. Mich15 N1-VASP protein. F NI using the reassortant virus H7N1Mich15. A, C, E, and F are shown as mean plus standard deviation.