FIG. 3.

Partial sequence alignment of the highly conserved regions between the four G. fujikuroi P450 monooxygenases involved in GA biosynthesis and three other cytochrome P450 monooxygenases: G.f. P450-1 → G. fujikuroi P450-1 (accession number (AC): Y15277); G.f. P450-2 → G. fujikuroi P450-2 (AC: Y15278); G.f. P450-3 → G. fujikuroi P450-3 (AC: Y15279); G.f. P450-4 → G. fujikuroi P450-4 (AC: Y17243); A.t. GA3 → ent-kaurene oxidase of Arabidopsis thaliana (AC: AF047719); H.s. P450 → human cholesterol 7α-monooxygenase (AC: P22680); C.m. P450 → alkane-inducible cytochrome P450 monooxygenase of Candida maltosa (AC: JS0726). The presumed heme-binding site of the P450-type enzymes is F--G---C-G.