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. 2001 Aug;67(8):3514–3522. doi: 10.1128/AEM.67.8.3514-3522.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 6 — Characterization of the P450-4-disruption mutant T12. (A) Total ion current traces from GC-MS analysis of culture filtrates of the P450-4-T12 transformant and wild type (IMI 58289). Peaks: 1, GA₉; 2, 7β-hydroxykaurenolide; 3, GA₄; 4, fujenoic acid; 5, GA₇; 6, GA₁₃; 7, 7β, 18-dihydroxykaurenolide; 8, GA₃. The traces are normalized to the largest peak in both traces. (B) HPLC radiochromatograms of extracts of mycelia and culture filtrates (medium) from incubations of ent-[¹⁴C]kaurene, ent-[¹⁴C]kaurenol, ent-[¹⁴C]kaurenal, and ent-[¹⁴C]kaurenoic acid with the wild-type (IMI 58289), P450-4-T12, and the mutant, B1-41a. (C) HPLC radiochromatograms of extracts of culture filtrates (medium) from incubations of ¹⁴C-labeled GA₁₂₋ aldehyde, GA₁₄, and GA₄ with the wild type and P450-4-T12.