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Characterization of SG139 transformed with the vector pP450-4-GC carrying the complete gene P450-4. (A) Northern blot analysis of SG139 and five complemented transformants. The blot was probed successively with the cDNA fragments of P450-4, cps/ks (control for the deletion), and the Gibberella 18S ribosomal DNA. (B) HPLC radiochromatograms of mycelial extracts from incubations of ent-[14C]kaurene with the wild type (IMI 58289), SG139, and SG139-transformant T10.
