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Expression of the P450 gene in the wild-type and P450-4 mutant strains. (A) RT-PCR using primers P450-4-RT1 and P450-4-RT2 with the following templates: genomic DNA from wild-type (lane 1), cDNA from wild-type (lane 2), and cDNA from B1-41a (lane 3). Lane 4 contains a 1-kb ladder as marker. (B) Northern blot analysis using the P450-4 and the cps/ks cDNAs as probes on RNA from wild-type and mutant strains.
