Figure 4.

Spatiotemporal control over MT dynamics in cell‐free systems by photocontrolling STEpo2. a, b) Schematic of in vitro MT dynamics imaging assay, and of kymographs with normal MT growth and catastrophes vs. patterns expected for STEpo2 as a 405 nm‐dependent 14‐protofilament MT stabiliser. c) No‐drug control kymograph showing MT shrinkage frequency and polymerisation restarts at the GMPCPP seed (405 nm illuminations in the purple dotted boxes). d) Time‐lapse images with two microtubules in the field of view (at left, 1 and 2), and their kymographs (at right). 405 nm illumination targeting microtubule 1 at 308 s within the purple box region of time‐lapse panel/kymograph (0.01 % of field of view) suppresses its catastrophes and creates a rescue zone far away from the seed.