Figure 5.

Spatiotemporal control over cellular MT dynamics with STEpo2. a–d) Live cell EB3‐GFP “comets” during GFP imaging with 488 nm (HeLa cells). MT inhibition with STEpo2 is initiated upon 405 nm illumination (cells first treated with 1 % DMSO, then imaged for 60 s for baseline, then photoactivated from time t=0 with 405 nm during imaging over 3 min; then an additional 0.6 μM E‐STEpo2 was applied, and 405 nm/imaging applied from time t*=0. 10 cells acquired). a) Mean±SD comet count (each cell normalised to t=0 of the DMSO control). b, c) Comet density statistics show large and significant differences upon STEpo2 photoactivation (dark at t=0 or t*=0, lit at t=60 s or t*=60 s; b shows pooled data, c shows longitudinal traces per cell; P‐values and μ mean differences as annotated refer to differences between treatments, pooling all cells). d) Stills from representative movie (data related to Movie S2).