Table 2.
Primer description
| Oligo name | Sequence 5′‐3′ | T m (°C) | GC‐content (%) |
|---|---|---|---|
| Meso 90F | CGTTTAGAATTGGGWAAYCCTG | 57.5 | 43.2 |
| Meso 308F I | ATTAATATTACTAATTTCWAGWATATTT | 50.5 | 10.7 |
| Meso 308F II | ATTAATACTACTGATTTCAAGWATATTT | 53.4 | 17.9 |
| Meso 518R | GGAATATTTTCWATTTTAWRAATTT | 49.0 | 14 |
| Platy 92F | ATTAGAATTAGGGACACCTTC | 54.0 | 38.1 |
| Platy 225F I | TTARTTCCTTTAATAWTATC | 44.0 | 17.5 |
| Platy 225F II | CTTRTACCATTAATATTRTC | 47.0 | 25 |
| Platy 344R | CCTGCTCCAAAAATAYTWCTRT | 54.7 | 36.4 |
Note: T m, melting temperature; GC‐content, guanine and cytosine content.