FIGURE 3.

(a) Cresyl violet staining shows cortical layers (roman numerals) and underlying white matter (WM) in the normal cerebral hemisphere wall in relation to eight depth zones (a vertical white bar with Arabic numerals). The region delimited for counting of immunopositive neurons was a vertical rectangle of 500 μm width located between the lumen of the lateral ventricle and the surface of cerebral hemisphere at the level of the anterior commissure. Within the region, neurons immunopositive for (b) parvalbumin (PV), (c) calretinin (CR), or (d) neuropeptide Y (NPY) were counted. As indicated with white rectangles, for each of the counting regions, example areas with neurons immunopositive for PV (b1), CR (c1, c2), and NPY (d1, d2) are shown at higher magnification. Each bar shows 100 μm length