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. 2022 Jul 11;18(12):4744–4755. doi: 10.7150/ijbs.73057

Figure 5.

Figure 5

PAP-1 is essential for efficent SARS-CoV-2 replication. (A) siRNA knockdown of PP2A, PP2B, PP2C, PLPP4, and PLPP5, and (B) LPIN2 and LPIN3 were performed in Caco-2 cells before SARS-CoV-2 infection for 48 hours (multiplicity of infection, MOI = 0.1). The viral yields in the cell lysates were determined by RT-qPCR and normalized with human β-actin. (C and D) The individual siRNA knockdown efficiency and specificity of (C) LPIN2 and (D) LPIN3. Results are shown as % of scramble siRNA-treated. **, P<0.01; ***, P<0.001 (Student's t-test). (E) siRNA knockdown of LPIN2 and LPIN3 were performed in Caco-2 cells before SARS-CoV-2 infection for 8 hours (MOI = 0.1). (F) LPIN3 overexpression plasmid was transfected to Caco-2 cells before SARS-CoV-2 infection for 48 hours (MOI = 0.1). (G) Western blot showing the overexpression of LPIN3 protein.