FIG. 2.
Sensitivity of P. aureofaciens TX-1 detection with PCR primers 49f and 617r and hybridization with the P. aureofaciens TX-1 probe in turfgrass rhizosphere soil inoculated with dilutions of P. aureofaciens TX-1 cells. (A) Agarose gel electrophoresis of the 568-bp PCR product amplified from soil DNA. (B) Hybridization of the P. aureofaciens TX-1 probe to DNA blotted from the gel shown in panel A. Soils were inoculated with 2.5 × 109, 2.5 × 108, 2.5 × 107, 2.5 × 106, 2.5 × 105, 2.5 × 104, 2.5 × 103, 2.5 × 102, and 2.5 × 101 cells g−1 (lanes 1 to 9, respectively) or were not inoculated (lane 10), as described in the text. Lane 11, negative (no DNA) PCR control.