FIG. 4.

DGGE analysis of 16S rRNA genes after PCR amplification of DNA isolated from the turfgrass environment with primers 341-f and 534-r-GC. (A) Lanes 1 and 2, leaf canopy DNA without nightly applications of P. aureofaciens TX-1 (lane 1) and with nightly applications (lane 2); lane 3, amplified P. aureofaciens TX-1 DNA extracted from a pure culture. The arrow indicates the position of DNA that represents the bacteria displaced by P. aureofaciens TX-1. (B) DGGE of soil (lanes 1 and 2) and thatch (lanes 3 and 4) DNA samples. Lanes 1 and 3, untreated samples; lanes 2 and 4, samples treated with nightly applications of P. aureofaciens TX-1.