TABLE 1.

Comparison of P. aureofaciens TX-1 PCR primer and probe sequences to nucleotide sequences of test isolates^a

Isolate	Primer 49f sequence	Probe sequence	Primer 617r sequence
Pseudomonas aureofaciens	GAAGCTTGCTTCTCTTGAGb	GTACTTACCTAATACGTGAGT	CTCGCCAGTTTTGGATG
P. cepacia	GGTGCTTNCACCTNGGGCG	CCTTGGCTCTAATACAGCCGG	CCTGCCAGTCACCAATG
P. aeruginosa	GAAGCTTGCTCCTGGATTC	GCAGTAAGTTAATACCTTGCT	CTCAGTAGTTTNGGATG
P. putida	AGAGCTTGCTCTTCGATTC	GCATTAACCTAATACGTTAGT	CTTGCCAGTTTTGGATG
P. stutzeri ATCC 17587	GAAGCTTGCTCCATGATTC	GCAGTAAGTTAATACCTTGCT	CTTGCCAGTTTTGAAAG
P. viridiflava pv-5	GAAGCTTGCTTCTCTTGAG	GCAGTAACCTAATACGTTATT	CTTGCCAGTTTTGGATG
P. syringae pv. syringae	GGTACTTGTACCTGGTGGC	GCAGTTACCTAATACGTRTCT	CTTGCCAGTTTTGGATG
P. putida AC10R	AGAGCTTGCTCTTCGATTC	GCATTAACCTAATACGTTAGT	CTCGCCAGTTTTGGATG
P. fluorescens ATCC 17419	GAAGCTTGCTTCTCTTGAG	GCATTAACCTAATACGTTAGT	TCAGTCAGTTTTGAATG
P. multivorans ATCC 17478	GTGCTTGCACCTGGTGGCG	CCTTGGCTCTAATACAGTCGG	CCTGCCAGTCACAAATG
Comamonas acidovorans	GAAGCTTGCTTCTCTTGAG	GTACTTACCTAATACGTGAGT	CCATGCAGGTANAAATG
C. acidovorans ATCC 17407	TAACAGGTCTTCGGACGCT	GGCTTCTCCTAATACGAGAGG	CCATGCAGGTANAAATG
C. testosteroni ATCC 15668	GAAGCTTGCTTCTCTTGAG	GCCTGGGGCTAATATCCCCGG	GAATGTAGCAACTAATG
Ralstonia eutropha	ACTTCGGTCTGGCGGCGAG	GCGCTGGTTAATACCTGGCGT	CCTTGCAGTCACAAGCG

^aP. syringae pv. syringae, Alcaligenes sp. strain BR40, and a Ralstonia strain (species unknown) were also used as test organisms; however, the complete sequence alignment with primers 49f and 617r and the P. aureofaciens TX-1 probe was not determined. 

^bBoldface type indicates bases conserved in isolate and P. aureofaciens TX-1 probe and primer binding sites.