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Specificity and linearity of monoclonal antibody (mAb) on the concanavalin A (Con A) column with serially blended samples. mAb with non‐terminal mannose (flow‐through) was clearly separated from mAb with terminal mannose (bound) by the Con A column (A). Percentage bound peak on Con A column showed a linear trend when samples containing 0, 20, 40, 60, 80, and 100% β‐N‐acetylhexosaminidase–treated drug substances were injected (B)
