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. 2022 Jul 8;9:915896. doi: 10.3389/fvets.2022.915896

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Copyright © 2022 Chiocchetti, De Silva, Aspidi, Cunha, Gobbo, Tagliavia, Sarli and Morini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photomicrographs of cryosections of canine skin showing showing serotonin 1a receptor (5HT1aR) immunoreactivity (IR) in the tissues of the healthy dogs (CTRL) (a–c) and in the dogs with atopic dermatitis (AD) (d–f). (a–c) The white arrows indicate the DAPI-labeled nuclei of some keratinocytes of the suprabasal layer showing bright 5-HT1aR-IR. (d–f) In the AD dogs, the cell membrane of the more superficial keratinocytes showed very bright 5-HT1aR-IR whereas the germinative cells of the basal layer and the deepest cells of the suprabasal layer were either nearly 5-HT1aR negative or expressed very faint 5-HT1aR-IR. Bar, 50 μm.