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. 2022 Feb 15;45(4):1229–1241. doi: 10.1111/pce.14277

Figure 4.

Figure 4

CPuORFs function as conditional regulators of translation. Translational responses following (a) mannitol, (b) heat, or (c) thermospermine treatments relative to appropriate controls are shown. Data represents means ± SEM for HG17 CPuORFs (striped bars), the HG7a CPuORF19 (solid grey bars), the HG15 SAC51_CPuORF (solid black bars), and 35S:LUC (control reporter without CPuORF; white bars). For CPuORF46‐LUC the number of independent lines (n) = 51, 26 and 25 for control, mannitol and heat treatments, respectively. For CPuORF47‐LUC n = 22 for control, mannitol and heat, and n = 12 for thermospermine treatment. For OsCPuORF38‐LUC n = 19 for all treatments. For CPuORF19‐LUC n = 12 for all treatments. For 35S:LUC n = 12 for all treatments. For SAC51_CPuORF‐LUC n = 21 for all treatments. CPuORF, conserved peptide upstream open reading frame; HSD, honestly significant difference; LUC, luciferase. Significant differences between controls and treatments for each CPuORF reporter at *p < 0.05 and **p < 0.01 (Tukey HSD inference) [Color figure can be viewed at wileyonlinelibrary.com]