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. 2022 Jan 12;28(8):e202103995. doi: 10.1002/chem.202103995

Figure 1.

Figure 1

(A) Design for a 5′‐siRNA‐3WJ. (B) Design for a 3′‐siRNA‐3WJ. In (A and B), the antisense strand (AS) is blue, the sense strand (SS) is grey, an adapter (AD) is orange, and the 3WJ is black. Strands: C1, chimera 1; C2, chimera 2. (C) Non‐denaturing gel mobilities of Renilla luciferase‐targeting and control siRNA‐M2 constructs. The loading amount of each sample was matched at 1.67 pmol. RNA was stained with SYBR Gold Nucleic Acid Gel Stain, which detects double‐stranded nucleic acids more sensitively than single‐stranded nucleic acids. [16] (D) Denaturing gel mobilities of Renilla luciferase‐targeting and control siRNA‐M2 constructs following incubation with (+) or without (−) recombinant human Dicer for 1 h at 37 °C. The 25‐ and 22‐nucleotide (nt) labels mark the expected range of Dicer cleavage products. The gel is representative of three independent replicates.