Figure 2.
(A) Activity of Renilla luciferase‐targeting siRNA‐M2 constructs on a Renilla luciferase reporter in 293T cells. (B) Activity of Renilla luciferase‐targeting siRNA‐M2 constructs on the same reporter in Dicer‐deficient (NoDice) cells. [23] (C) Activity of control siRNA‐M2 constructs on a Renilla luciferase reporter in 293T cells. (D) Activity of control siRNA‐M2 constructs on the same reporter in NoDice cells. [23] In (A–D), siREN and siRND served as positive and negative controls. Treatments were transfected at 0, 2.5, 10, and 40 nM. Data are mean Renilla/Firefly values normalized to the 0 nM treatment ±SD (n=3). Statistics are two‐way ANOVA with Dunnett's multiple comparisons test against the 0 nM treatment: *P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001.