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Thermal stability of the (A) FDH part of the fusion protein with 50 mM phosphate buffer, pH 7 containing 125 mM formate and 1 mM NAD+ and the (B) azoreductase part of the fusion protein using 50 mM phosphate buffer, pH 7 containing 150 μM NADH, 50 μM FMN, and 25 μM (1). The protein was incubated in different temperatures and the activity was measured from different time points of incubation up to 2 h. Results for 50 °C was not shown since the enzymes were rendered inactive after 1 h.
