Table 1.

Kinetic parameters of FDH_C23S activity from Candida boidinii and the FDH activity of FDH_C23S+AzoRo at 25 °C. The reaction mixture (1 mL) contained 2.6 μg of enzyme in a 50 mM phosphate buffer, pH 7 with varying mixture of either NAD⁺ or formate.

	Substrate	K M [mM]	k cat [s−1]	k cat/K M [mM/s]
FDHC23S	Formate[a] NAD+[b]	4.23±0.07 0.05±0.02	1.78±0.04 1.93±0.12	0.42±0.06 35.8±5.32
FDHC23S + AzoRo	Formate[a] NAD+[b]	48.9±4.88 0.08±0.02	1.80±0.06 1.37±0.04	0.04±0.01 16.3±2.34

[a] This indicates that the formate was varied (5 mM to 200 mM) with a constant concentration of 1 mM NAD⁺ in the reaction. [b] This indicates the NAD⁺ was varied (0.1 mM to 1 mM) with a constant concentration of 125 mM formate in the reaction.