Fig. 1.

Upregulation of NOX2 and its regulatory proteins is associated with excessive H₂O₂ generation in PD toxins-induced neuronal cells. PC12 cells and primary neurons were treated with 6-OHDA (30, 60 and/or 120 μM), MPP⁺ (0.5, 1 and/or 1.5 mM) or rotenone (0.5, 1 and/or 2 μM) for 6, 12 and/or 24 h, as described in Materials and Methods. A and B Total cell lysates were subjected to western blotting using indicated antibodies. The blots were probed for β-tubulin as a loading control. Similar results were observed in at least three independent experiments. C Intracellular H₂O₂ was imaged (in green) using a peroxide-selective probe H₂DCFDA. Scale bar: 20 μm. D and E Fluorescent intensity of intracellular H₂O₂ imaging was quantified. Results were presented as mean ± SEM, n = 5. **P < 0.01, difference with control group.