Fig. 6.

Ectopic expression of constitutively active Akt attenuates upregulation of NOX2 and its regulatory proteins, generation of H₂O₂, as well as apoptosis in PD toxins-induced neuronal cells. PC12 cells, infected with Ad-myr-Akt or Ad-LacZ (as control), respectively, were treated with/without 6-OHDA (120 μM), MPP⁺ (1 mM) or rotenone (1 μM) for 24 h. A Total cell lysates were subjected to western blotting using indicated antibodies. The blots were probed for β-tubulin as a loading control. Similar results were observed in at least three independent experiments. B Intracellular H₂O₂ was imaged and quantified using a peroxide-selective probe H₂DCFDA. C Apoptotic cells were evaluated by nuclear fragmentation and condensation using DAPI staining. Results were presented as mean ± SEM, n = 5. ^aP < 0.05, difference with control group; ^bP < 0.05, Ad-myr-Akt group vs Ad-LacZ group.