FIG. 5.

Delineation of the putative BK5-T origin of replication. The thick solid bar represents the internal orf49 sequence generated by PCR that contained the putative ori. Horizontal arrows shown underneath the bar indicate the positions of the four repeat sequences (DR1 to DR3 and IR1). The plasmid designations of the clones containing deletion fragments are listed on the left and the EOP values (averaged from at least four different experiments) are given on the right. The horizontal thin lines indicate the DNA fragments cloned in the plasmid constructs. The plasmids were introduced into lactococcal host strain H2 (Table 1).