Patient sampling items	Patient characteristics and setting items	Index test items	Reference standard items	Flow and timing items	Notes items
A1 Purpose	B1 Setting	D1.1 Test name (please include product code if reported)	E1 Reference standard for cases including threshold	F1 What was the time interval between index and reference tests?	G1 Funding
A2 Design (and description of groups labelled [1] [2] …)	B2 Location (include name of institution if available)	D1.2 Manufacturer	E1.1 PCR genetic targets	F2 Did all patients receive the same reference standard?	G2 Publication status
A3 Recruitment	B3 Country	D1.3 Antigen or genetic target	E2 Samples used	F3 Missing data	G3 Source (preprint or journal name)
A4 Were cases recruited prospectively or retrospectively?	B4 Dates	D1.4 Antibodies used	E3 Timing of reference standard	F4 Uninterpretable result s	G4 Study author CoI (including any manufacturer affiliations)
A5 Sample size (virus/COVID cases)	B5 Symptoms and severity	D1.5 POC or laboratory	E4 Was it blind to index test?	F5 Indeterminate results (index)	G5 Comment
A6 Inclusion and exclusion criteria	B6 Demographics	D1.6 Assay format	E5 Did it incorporate index test?	F5.1 Indeterminate results (reference)
A7 Comment	B7 Exposure history	D1.7 When were samples taken?	E6 Reference standard for non‐cases	F6 Samples or patients
	B8 Comment	D1.8 Samples used (include who collected by)	E7 Samples used	F7 Comment
	Non‐COVID patients (if additional groups)	D1.8.1 Transport media (volume and manufacturer detail)	E8 Timing of reference standard
	C1.1 Group name	D1.8.2 Sample storage and timing of test	E9 Was it blind to index test?
	C1.2 Source and time	D1.9 Who applied the test (include reported training/e)?	E10 Did it incorporate index test?
	C1.3 Characteristics	D1.10 How was positive defined?	E11 Comment
	C2.1 Group name	D1.11 Blinded to reference standard
	C2.2 Source and time	D1.12 Threshold predefined
	C2.3 Characteristics	D1.13 Comment
CoI: conflict of interest; POC: point of care; PCR: reverse transcription polymerase chain reaction