FIGURE 5.

The three catalytic subunits of PI3K immunoprecipitate with IGF‐1R in male and females but have different expression levels between sexes. Primary astrocyte cultures were stimulated with IGF‐1 during 45 min in order to activate the IGF‐1R and promote the formation of IGF‐1R/PI3K complex. Cell lysates were immunoprecipitated with IGF‐1‐R antibody or normal rabbit IgG and then immunoblotted with the indicated antibodies. N = 5–9 independent cultures. (a) Representative image showing lane 1—IGF‐1R co‐IP positive control, lanes 3, 5 y 7—p110α, β, and δ co‐elutes with IGF‐1R. WCE, whole‐cell extract, IGF‐1R‐IP, IGF‐1R co‐immunoprecipitation. (b) Quantitative analysis of the p110α, β, and δ and IGF‐1R expression levels evaluated in the whole‐cell extract of male and female astrocytes. Data are presented as means ± SEM, **p < .01 indicates basal sex differences measured by unpaired two‐tailed t test