FIGURE 6.

Effect of selective inhibitors of PI3K isoforms on IGF‐1 modulation of male astrocytic inflammatory response. PI3K pathway was blocked with isoform‐selective PI3K inhibitors in astrocytes treated with LPS + IGF‐1. Graphs show the expression of IL‐1β, IL‐6, and IL‐10 mRNA quantified by q‐RTPCR. Note that only the p110α inhibitor AG66 significantly suppressed the IGF‐1 effect on the IL‐6 and IL‐10 mRNA expression in male astrocytes, whereas TGX‐221 and CAL‐101 had no effect, suggesting that p110α subunit drives IGF‐1 modulation of astrocytic inflammatory response. Data are presented as means ± SEM. Significant differences **p < .01, effect of inhibitors versus LPS and ^# p < .05, ^## p < .01 versus LPS + IGF‐1 treated groups, measured by ordinary one‐way ANOVA followed by Tukey's multiple comparisons post hoc test. N = 6 independent cultures