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SDS-PAGE to show the overexpressed recombinant proteins in three clones. (A) Lanes 1 and 2, clone with nidA-pBAD/Thio plasmid; lane 3, clone with nid(B+A)-pBAD/Thio plasmid. (B) Clone with nidD- pBAD/Thio plasmid. Lane M, protein size marker; lane 1, sample from the supernatant after sonication of the cells; lane 2, recombinant proteins partly purified by using an Ni-NTA spin column. Arrows, locations of target proteins.
