TABLE 1.
Bacterial strains and plasmids
| Strain, phage, plasmid, or vector | Description | Source or reference |
|---|---|---|
| Strains and phage | ||
| Mycobacterium sp. strain PYR-1 | Mineralizes PAHs, such as fluoranthene, pyrene, phenanthrene, etc. | 14–16 |
| E. coli XL1-Blue MRF′ | Lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] | Stratagene |
| E. coli XLOLR | Lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] Su−(nonsuppressing) λr | Stratagene |
| ExAssist helper phage | For in vivo excision of the pBK-CMV phagemid from ZAP Express vector with E. coli XLOLR | Stratagene |
| E. coli TOP10 | One Shot-competent cells; F′ mcrA Δ(mrr-hsdRMS-mcrBC)Φ80lacZΔM15 Δ(ara-leu)7697 | Invitrogen |
| E. coli JM 109 | Competent cells; F′ proAB laclqZΔM15 | Promega |
| Plasmids and vectors | ||
| Zap Express vector | Lambda vector; prokaryotic and eukaryotic expression, in vivo excision of pBK-CMV vector | Stratagene |
| pBK-CMV vector | Neor Kanr; colE1 origin; lacZ; CMVa promoter | Stratagene |
| My6-pBK-CMV phagemid | pBK-CMV phagemid with 5.2-kb insert from Mycobacterium sp. strain PYR-1 | This study |
| pBAD/Thio-TOPO vector | Ampr; pMB1 origin; Six-His-thioredoxin ORF; arabinose induced | Invitrogen |
| nidA-pBAD/Thio plasmid | pBAD/ThioFusion with nidA gene | This study |
| nid(B+A)-pBAD/Thio plasmid | pBAD/ThioFusion with nidB plus nidA genes | This study |
| nidD-pBAD/Thio plasmid | pBAD/ThioFusion with nidD gene | This study |
a
CMV, cytomegalovirus.