Table 1.
Analysis of the polar variants in which different amino acids exchanged to polar residue towards three OSs resistance in BSLA–SSM library.[a]
|
Exchange |
Fraction of substitution [%] |
|||
|---|---|---|---|---|
|
|
beneficial |
unchanged |
decreased |
inactive |
|
polar to polar |
4.0–11.1 |
74.4.2–83.1 |
3.0–7.3 |
7.3–12.6 |
|
aromatic to polar |
4.8–14.3 |
66.7–77.4 |
4.6–8.3 |
11.9–14.3 |
|
aliphatic to polar |
2.9–14.0 |
60.4–72.1 |
7.1–17.2 |
8.4–18.5 |
|
charged to polar |
2.0–7.4 |
62.6–78.8 |
2.0–16.7 |
13.8–16.3 |
[a] 22 % (v/v) DOX, 60 % (v/v) DMSO, and 12 % (v/v) TFE were used; amino acid is classified as follows: aromatic: F, Y, W; aliphatic: A, V, L, I, G; polar: C, M, P, S, T, N, Q; charged: D, E, K, H, R.