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. Author manuscript; available in PMC: 2022 Jul 22.
Published in final edited form as: Nat Cell Biol. 2022 Mar 7;24(3):353–363. doi: 10.1038/s41556-022-00853-8

Extended Data Fig. 2. Octyl-ITA (OI) can enter the cells and be hydrolyzed to itaconate.

Extended Data Fig. 2

a, OI can enter and accumulate in the cell. RAW264.7 cells were treated with different concentrations of OI for different lengths of time, followed by LC-MS/MS analysis of intracellular ITA. Data shown represent average values with S.D. of three independent experiments.

b, Unmodified ITA can enter and accumulate in the cell. RAW264.7 cells were treated with increased concentrations of ITA as indicated, and the intracellular concentration of ITA and succinate was measured by LC-MS/MS. Data shown represent average values with S.D. of four independent experiments.

c, Intracellular accumulation of cell-permeable metabolites. HEK293T cells were treated with cell-permeable octyl-ITA and octyl-L-2-HG for 12 hours as indicated, and the intracellular levels of L-2HG and ITA were quantified by LC-MS/MS. Shown are average values of 2 independent experiments. The intracellular concentrations of indicated metabolites are shown.