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. 2022 Jul 22;22:189. doi: 10.1186/s12902-022-01103-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Metformin enhances the osteogenic differentiation ability of MC3T3-E1 cells under HG conditions. A ARS was applied for the assessment of calcification in MC3T3-E1 cells in each group. B ALP colorimetric assay kit was utilized to measure the activity of ALP in each group of cells; C/D Western blot was performed for the detection of the expression of osteogenic differentiation-related genes (Runx2, OCN) in each group of cells, **P < 0.01, vs. Control; ##P < 0.01, vs. HG