TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| Terrabacter sp. strain DBF63 | DF+a | 21 |
| Pseudomonas sp. strain CA10 | CAR+b | 24 |
| E. coli JM109 | recA1 Δ(lac-proAB) endA1 gyrA96 thi-1 hsdR17 relA1 supE44 [F′ traD36 proAB laqIqZ ΔM15] | 40 |
| Plasmids | ||
| pUC119 | AprlacZ; pMB9 replicon | 40 |
| pSTV28 | CmrlacZ; pMB9 replicon | Takara Shuzo Co., Ltd. |
| pUCA1 | Apr; pUC119 containing 6.9-kb EcoRI insert of CA10 DNA | 29 |
| pDF32 | Apr; pUC119 containing 2.6-kb BglII-SphI insert of DBF63 DNA | 16 |
| pUCARA | Apr; pUC119 containing 5.6-kb EcoRI insert of pUCA1 with the deletion of 1.3-kb PstI-BglI fragment | 28 |
| pLM1RM | Cmr; pSTV28 containing 3.0-kb BamHI insert of DBF63 DNA | 15 |
a
DF+, able to use dibenzofuran as the sole carbon or energy sources.
b
CAR+, able to use carbazole as sole carbon, nitrogen, and energy sources.