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Distribution of fluorescently labelled transport receptors (Impβ) in the human NPC measured in permeabilized cells. Transport protein binding is associated with specific FG nups (Nup153 and Nup358) at the opposite sides of the transport barrier. In a mutant cell lacking Nup153, transport protein accumulation is severely diminished, especially in the nuclear basket. RanGTP modulates NPC occupancy. Reproduced from [43], permission pending.
