TABLE 2.
Preliminary microbiological characterization of subsamples from a black smoker chimney at the PACMANUS sitea
| Subsample no. | Total amt (g) | Cell density (cells/g [wet wt]) | DNA yield (ng/g [wet wt]) | RNA yield (ng/g [wet wt]) | rRNA/rDNA composition (%) for:
|
|
|---|---|---|---|---|---|---|
| Bacteria | Archaea | |||||
| I | 30 | 1.0 × 106 | 5.1 | 2.2 | 78.3/92.6 | 19.9/7.4 |
| II | 15 | 3.2 × 107 | 169 | 100 | 66.8/94.7 | 30.5/5.3 |
| III | 36 | 4.5 × 105 | 3.2 | 1.3 | 97.6/100 | 1.8/ND |
| IV | 18 | 4.5 × 105 | 1.6 | 1.4 | 98.5/97.2 | 2.8/2.8 |
| V | 19.5 | 8.0 × 105 | 3.0 | 4.5 | 95.1/100 | 2.2/ND |
| VI | 15 | 5.1 × 105 | 1.6 | 1.7 | 76.7/92.8 | 23.1/7.2 |
a
Prokaryotic universal, bacterial, and archaeal SSU rRNA/rDNA composition was quantified by using RNA dot hybridization or quantitative fluorogenic PCR (52). The proportions represent the result by RNA dot hybridization divided by the result by quantitative fluorogenic PCR. ND, not detected.