TABLE 3.
Distribution of representative T-RFLP ribotypes and clone types of archaeal rDNA obtained from a black smoker chimneya
| Clone type (T-RFLP ribotype) | No. of archaeal rDNA clones characterized per subsample:
|
|||||
|---|---|---|---|---|---|---|
| I | II | III | IV | V | VI | |
| MHVG-1 | ||||||
| pPACMA-Y (9) | 1 | 3 | 0 | 0 | 0 | 0 |
| Crenarchaeota | ||||||
| Igniococcales | ||||||
| pPACMA-I (5) | 7 | 2 | 0 | 11 | 0 | 0 |
| pPACMA-X (5) | 13 | 4 | 0 | 25 | 3 | 0 |
| Euryarchaeota | ||||||
| Thermococcales | ||||||
| pPACMA-C (6) | 19 | 25 | 29 | 0 | 0 | 0 |
| pPACMA-A (6) | 0 | 0 | 9 | 0 | 0 | 0 |
| pPACMA-B (6) | 0 | 0 | 2 | 0 | 0 | 0 |
| Unknown group | ||||||
| pPACMA-N (8) | 1 | 3 | 0 | 0 | 2 | 0 |
| DHVEG | ||||||
| pPACMA-M (10) | 0 | 5 | 0 | 0 | 0 | 0 |
| pPACMA-Q (10) | 2 | 0 | 1 | 0 | 0 | 0 |
| pPACMA-W (7) | 0 | 1 | 1 | 0 | 0 | 0 |
| pPACMA-P (7) | 1 | 2 | 2 | 0 | 0 | 0 |
| pPACMA-V (7) | 0 | 3 | 1 | 0 | 0 | 0 |
| Halobacteriales | ||||||
| pPACMA-H (3) | 0 | 0 | 0 | 0 | 9 | 21 |
| pPACMA-T (3) | 0 | 0 | 0 | 0 | 0 | 7 |
| pPACMA-J (4) | 0 | 0 | 0 | 1 | 0 | 0 |
| pPACMA-L (3) | 0 | 0 | 0 | 1 | 5 | 8 |
| pPACMA-U (4) | 0 | 0 | 0 | 0 | 0 | 5 |
| pPACMA-K (4) | 0 | 0 | 0 | 2 | 0 | 0 |
| pPACMA-E (4) | 0 | 0 | 0 | 3 | 31 | 10 |
| pPACMA-S (4) | 0 | 0 | 0 | 0 | 1 | 0 |
| pPACMA-G (4) | 0 | 0 | 0 | 1 | 0 | 0 |
| pPACMA-F (4) | 0 | 0 | 0 | 0 | 13 | 2 |
| Total | 44 | 48 | 45 | 44 | 65 | 53 |
a
Composition of archaeal rDNA clone types was determined by partial sequencing analysis of rDNA clones of archaeal primer PCR libraries.