TABLE 4.
Viable-cell counts of Thermococcus members and halophilic microorganisms calculated by three-tube MPN cultivation methoda
| Subsample no. | Viable-cell count in:
|
||||
|---|---|---|---|---|---|
| MJYPS medium for Thermococcus (cells g [wet wt]−1)
|
HA medium for Haloarcula (cells g [wet wt]−1)
|
||||
| 55°C | 75°C | 90°C | 30°C | 45°C | |
| I | 4.6 × 102 | 2.4 × 102 | 3.9 × 101 | ND | ND |
| II | 2.1 × 104 | 1.5 × 104 | 9.0 × 103 | ND | ND |
| III | 7.5 × 102 | 4.3 × 102 | 7.5 × 101 | ND | ND |
| IV | 9.0 × 100 | 9.0 × 100 | ND | 9.3 × 101 | ND |
| V | ND | ND | ND | 9.3 × 102 | 9.3 × 101 |
| VI | ND | ND | ND | 7.5 × 102 | 9.0 × 101 |
a
Viable-cell counts obtained by the MPN method using HA medium represent not Haloarcula but halophilic bacteria such as Halomonas spp, as described in Results. ND, not detected.