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. 2022 Apr 20;28(14):3116–3126. doi: 10.1158/1078-0432.CCR-22-0421

Figure 2.

Figure 2. Integrin αIIbβ3 is crucial for the TC signaling pathway. A, Lysates from NIH3T3 cells transfected with FLAG-CTGF were IP with an anti-FLAG antibody. Coimmunoprecipitated integrins were detected in an immunoblot using anti-integrin αIIb, anti-integrin β3, or anti-integrin αV antibodies. B, Immunoblots to detect the efficiency of shRNA-mediated knockdown of integrin β3, αV, or αIIb in NIH3T3/TC cells. SHC1 is an EV, SHC2 is a scrambled control shRNA. For integrin knockdowns, two independent shRNA were used. C, Quantification of the soft agar colony counts after knockdown of the indicated integrins in NIH3T3/TC cells. Silencing of integrin αIIb and integrin β3, but not integrin αV, inhibited the ability of NIH3T3/TC cells to form colonies. Data represent mean and SD of individual wells. IP, immunoprecipitated; IB, immunoblot.

Integrin αIIbβ3 is crucial for the TC signaling pathway. A, Lysates from NIH3T3 cells transfected with FLAG-CTGF were immunoprecipitated with an anti-FLAG antibody. Coimmunoprecipitated integrins were detected in an immunoblot using anti-integrin αIIb, anti-integrin β3, or anti-integrin αV antibodies. B, Immunoblots to detect the efficiency of shRNA-mediated knockdown of integrin β3, αV, or αIIb in NIH3T3/TC cells. SHC1 is an EV, SHC2 is a scrambled control shRNA. For integrin knockdowns, two independent shRNA were used. C, Quantification of the soft agar colony counts after knockdown of the indicated integrins in NIH3T3/TC cells. Silencing of integrin αIIb and integrin β3, but not integrin αV, inhibited the ability of NIH3T3/TC cells to form colonies. Data represent mean and SD of individual wells. IP, immunoprecipitated; IB, immunoblot.