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Sites in the P. aeruginosa aliphatic amidase AmiE at which mutations improved growth of E. coli in the presence of isobutyramide as a sole nitrogen source. The active site is marked by a space-filled ligand covalently attached to the active site Cys166. Colors indicate the number of substitutions that were found to be favorable at each position in the protein. Reproduced from Nat Commun. 8:15695, 2017 under a Creative Commons license (http://creativecommons.org/licenses/by/4.0/).
